NEU259: Introduction to Light and Electron Microscopy (Spring 2009)

Instructors:
Maryann Martone, Ph. D.; mmartone@ucsd.edu, X20745
Gina Sosinsky, Ph. D.; gsosinsky@ucsd.edu, X40128
Mark Ellisman, Ph. D.; mellisman@ucsd.edu, X41392

Time and Location: Tuesday/Thursday 10:00 - 11:00 am, 1000 BSB Conference Room
Thursday demo: 11:00 - 12:30, Location to be announced

Syllabus: (document download)

Readings: Readings will be assigned by instructor and will be available with our administrative assistants for the NCMIR facility. A web course on electron microscopy can be found at http://em-outreach.ucsd.edu.
Our course websites can be accessed at: http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm

 

No.	Date	Topic	Lecturer	Demo
1	31-Mar	Intro to Course / Intro to Optics I	Maryann Martone/ James Bouwer
2	2-Apr	Intro to Optics II	James Bouwer	LM - MM/TD
3	7-Apr	Confocal Microscopy/Fluorescence	Tom Deerinck
4	9-Apr NOTE:	Transmission Electron Microscopy LECTURE ROOM CHANGE THIS DATE ONLY:	Tom Deerinck LEICHTAG 2A05	TEM – TD
5	14-Apr	Multiphoton Microscopy	James Bouwer
6	16-Apr	Optical probes for live imaging	Guido Gaietta	NCMIR Tour-ME
7	21-Apr	Specimen Preparation	Maryann Martone	Confocal – Hiro Hakozaki
8	23-Apr	Immunocytochemistry for LM and EM	Maryann Martone
9	28-Apr	In Situ Hybridization	Armin Blesch
10	30-Apr	Midterm		No Demo
11	5-May	Advance Light Microscopy Techniques (e.g. TIRF, near field)	Hiro Hakozaki
12	7-May	Digital Imaging	James Bouwer	TBA
13	12-May	Image Processing/Analysis Fundamentals	Gina Sosinsky
14	14-May	Deconvolution Microscopy	James Bouwer	Adobe Photoshop-TD Deconvolution Demo ImageJ (Hakozaki)
15	19--May	Meso-scale imaging techniques	Mark Ellisman
16	21-May	Electron Tomography	Guy Perkins	Tomography
17	26-May	Molecular Microscopy: Specimen Prep	Norm Olson
18	28-May	Molecular Microscopy: 3D Recon Methods	Tim Baker	CryoEM (Olson)
*19	2-June	Scanning Probe Microscopies	Gina Sosinsky	SEM demo (tentative)
20	4-Jun	OPEN
** Finals Week	9-Jun	Future of Microscopy & Student Presentations	All instructors

 

* Written assignment "My thesis project and how microscopy cna contribute to my thesis." (1 page limit is for the body of writing. Use Arial font, 11 pt, 1 inch margins (2.5 cm) relative to 8.5 x 11 inch paper format. Line spacing should be 6 lines/inch (use single spacing with Microsoft Word).

**Final examination is take-home and due back by June 11th.