NCMIR is a Biomedical Technology Research Center (P41) supported by the National Institute for General Medical Sciences (NIGMS) of the National Institutes of Health (NIH). The center fulfills its mission through technology development, collaboration, service, training, and dissemination. NCMIR aims to develop preparative methods and analytical approaches to 3D microscopy applicable to neurobiology and cell biology, incorporating equipment and implementing software that expand the analysis of 3D structure. The core research activities in the areas of specimen development, instrument development, and software infrastructures maximize the advantages of higher voltage electron microscopy and correlated light microscopies to make ambitious imaging studies across scales routine, and to facilitate the use of resources by biomedical researchers. NCMIR actively recruits outside users who will not only make use of these resources, but who also will drive technology development and receive training.