Protocols

NCMIR protocols are managed and openly disseminated via Protocols.io.  Access protocols here.

List of protocols available for download (as of Oct 1., 2023):

  • Photo-oxidation using MiniSOG with EM Preparation of Transfected Culture Cells  

  • Preparation of Biological Tissues for Serial Block Face Scanning Electron Microscopy (SBEM)

  • Preparation of Cultured Cells for Serial Block Face Scanning Electron Microscopy (SBEM)

  • Correlative Microscopy for Localization of Proteins in Situ: Pre-embedding Immuno-Electron Microscopy Using FluoroNanogold, Gold enhancement, and Low-Temperature Resin

  • Ln-DAB2 Solutions

  • Lanthanum DAB metals, Ln-DAB2 labeling of APEX2

  • Creating Gelvatol Solution

  • Karnovsky’s Modified Fixative

  • 0.3M Sodium Cacodylate Buffer pH 7.4 Stock Solution Recipe

  • Ringer’s Solution

Please email any questions regarding protocols/SOPs to This email address is being protected from spambots. You need JavaScript enabled to view it.